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Image Search Results
Journal: Respiratory Research
Article Title: Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis
doi: 10.1186/s12931-024-02740-2
Figure Lengend Snippet: Bhlhe40 deficiency alleviates GSDMD-mediated pyroptosis and caspase-1 and caspase-11 pathways in LPS-induced ALI mice. ( A ) Representative Western blot of GSDMD FL and GSDMD NT in the lung tissues of mice. ( B ) Representative Western blot of cleaved IL-1β in the lung tissues of mice. ( C ) Representative images of F4/80 and GSDMD NT double-immunofluorescence staining of LPS-induced lungs. Scale bar = 50 μm and 20 μm. (D) Representative Western blot of pro-caspase-1, cleaved caspase-1, pro-caspase-11, cleaved caspase-11, NLRP3, ASC, TLR4 and MYD88 in the lung tissues of mice. n = 6
Article Snippet: Antibodies used for Western blot, immunohistochemistry and Immunofluorescence included Bhlhe40 (NB100-1800SS) from Novus (Centennial, CO, United States), GAPDH (ab181602), GSDMD (ab209845), Caspase-11 (ab180673) from Abcam (Cambridge, United Kingdom), F4/80 (70076T), NLRP3 (15101 S), ASC (67824T) from Cell Signal Technology (Danvers, MA, United States),
Techniques: Western Blot, Double Immunofluorescence Staining
Journal: Respiratory Research
Article Title: Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis
doi: 10.1186/s12931-024-02740-2
Figure Lengend Snippet: Bhlhe40 deficiency inhibits GSDMD-mediated pyroptosis through both canonical and non-canonical pathways in BMDMs and in RAW264.7 cell line. ( A - C ) Representative Western blot of GSDMD FL , GSDMD NT , cleaved IL-1β, pro-caspase-1, cleaved caspase-1, pro-caspase-11, cleaved caspase-11, NLRP3 and ASC in BMDMs. ( D - E ) RAW264.7 cells were transfected with three Bhlhe40 siRNAs (siRNA#1, siRNA#2 and siRNA#3) or normal control siRNA (NC) for 48 h. The protein levels of Bhlhe40 were detected by western blots and quantified analysis. ( E - F ) Representative Western blot of GSDMD FL , GSDMD NT , cleaved IL-1β, pro-caspase-1, cleaved caspase-1, pro-caspase-11, cleaved caspase-11, NLRP3 and ASC in RAW246.7 cells. n = 3. Data are shown as the mean ± SEM. Statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test. *p < 0.05, ns p > 0.05
Article Snippet: Antibodies used for Western blot, immunohistochemistry and Immunofluorescence included Bhlhe40 (NB100-1800SS) from Novus (Centennial, CO, United States), GAPDH (ab181602), GSDMD (ab209845), Caspase-11 (ab180673) from Abcam (Cambridge, United Kingdom), F4/80 (70076T), NLRP3 (15101 S), ASC (67824T) from Cell Signal Technology (Danvers, MA, United States),
Techniques: Western Blot, Transfection, Control